## Abstract During Chinese hamster ovary (CHO) cell culture for foreign protein production, cells are subjected to programmed cell death (PCD). A rapid death at the end of batch culture is accelerated by nutrient starvation. In this study, type II PCD, autophagy, as well as type I PCD, apoptosis, w
Autophagy and its implication in Chinese hamster ovary cell culture
โ Scribed by Yeon Jung Kim, Eric Baek, Jae Seong Lee, Gyun Min Lee
- Book ID
- 120967525
- Publisher
- Springer Netherlands
- Year
- 2013
- Tongue
- English
- Weight
- 537 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Two degradative activities were found in a recombinant Chinese hamster ovary cell culture. These activities became more dominant under high cell density and extended running time, as achieved in a semi-continuous perfusion culture. The first, insulin degradative activity caused a growth upset in the
One of the main problems in the culture of Chinese Hamster Ovary (CHO) cells continues to be the inability to maintain the viability of the cultures over an extended period of time. The rapid decline in viability at the end of the culture is exacerbated by the absence of serum. In trying to reduce t