## Abstract Upon nutrient deprivation during culture, recombinant Chinese hamster ovary (rCHO) cells are subjected to two types of programmed cell death (PCD), apoptosis and autophagy. To investigate the effect of Bclβx~L~ overexpression on apoptosis and autophagy in rCHO cells, an erythropoietin (
β¦ LIBER β¦
Autophagy and apoptosis of recombinant Chinese hamster ovary cells during fed-batch culture: Effect of nutrient supplementation
β Scribed by Young Kue Han; Tae Kwang Ha; So Jeong Lee; Jae Seong Lee; Gyun Min Lee
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 758 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0006-3592
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The application of a stoichiometric medium design approach was studied in fed-batch cultivation of Chinese hamster ovary (CHO) cells. A serum-free medium containing a very low protein concentration (2 mg/L insulin) was developed. A supplemental medium was formulated according to the stoichiometric e