Autonomous regulation of growth cone filopodia

✍ Scribed by Rehder, Vincent ;Cheng, Su

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 192 KB
• Volume: 34
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(19980205)34:2<179::aid-neu7>3.0.co;2-#

✦ Synopsis

The fan-shaped array of filopodia ber are regulated by intracellular calcium. Here, we is the first site of contact of a neuronal growth cone investigated filopodial dynamics and their regulation with molecules encountered during neuronal pathby the second-messenger calcium in growth cones finding. Filopodia are highly dynamic structures, and which were physically isolated from their parent neuthe ''action radius'' of a growth cone is strongly deterron by neurite transection. Our results show that isomined by the length and number of its filopodia. Since lated growth cones have longer but fewer filopodia interactions of filopodia with instructive cues in the than growth cones attached to their parent cell. These vicinity of the growth cone can have effects on growth isolated growth cones, however, are fully capable of cone morphology within minutes, it has to be assumed undergoing calcium-induced cytoskeletal changes, that a large part of the signaling underlying such morsuggesting that the machinery necessary to perform phological changes resides locally within the growth changes in filopodial length and number is fully intrincone proper. In this study, we tested the hypothesis sic to the growth cone proper. ᭧ 1998 John Wiley & Sons, that two important growth cone parameters -Inc. J Neurobiol 34: 179-192, 1998 namely, the length and number of its filopodia-are Keywords: Helisoma; intracellular calcium; calmoduregulated autonomously in the growth cone. We prelin; neurite transection viously demonstrated in identified neurons from the