✦ LIBER ✦

Automated synthesis of double dye-labeled oligonucleotides using tetramethylrhodamine (TAMRA) solid supports

✍ Scribed by Bashar Mullah; Alex Andrus

Book ID: 104257640
Publisher: Elsevier Science
Year: 1997
Tongue: French
Weight: 204 KB
Volume: 38
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(97)01286-0

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✦ Synopsis

TAMRA fluorescent dye-labeled non-nucleasidic synthesis supports have been derivatized for automated synthesis of 3" dye-labeled oligonucleotides. I Synthesis with 5 'fluorescent dye phasphoramidites leads to 5' and 3' double labeled oligonucleotides, useful in Taqman assay and FRET experiments. Cleavage and deprotection of dye-labeled oligonucleotides ore carried aut with tert-butylamine:methanol:water (1:1:2) without any degradation and~or modification of dyes and nucleobases.

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Very fast (4 sec) attachment of nucleosides to CPG supports, via succinyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU, HBPipU) or phospbonium (BOP, PyBOP ®, Py-BroP, BroP) coupling reagents and automated derivatization of CPG and polystyrene supports just prior to oligonucleot