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Automated quantitative assay for hydroxylysine in biological materials

✍ Scribed by Nelly Blumenkrantz; Gustav Asboe-Hansen

Book ID
108313783
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
352 KB
Volume
8
Category
Article
ISSN
0009-9120

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πŸ“œ SIMILAR VOLUMES

Quantitative assay for hydroxylysine in
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Collagen and related glycoproteins are the only animal proteins which contain significant amount's of the hydroxyamino acids hydroxyproline and hydroxylysine. A number of chemical procedures for the quantitative assay for hydroxyproline have been developed, and these assay procedures have been used

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Diclofenac and an internal standard are isolated from biological samples by acid-specific extraction and converted into the corresponding indolones with 0.5% sulphuric acid in 2,2,2-trifluorethanol. The neutral derivatives are isolated by solvent extraction and subjected to gas-liquid chromatography