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Automated quantitative analysis of wood carbohydrates by borate complex ion exchange chromatography

โœ Scribed by M. Sinner; M. H. Simatupang; H. H. Dietrichs

Publisher
Springer
Year
1975
Tongue
English
Weight
958 KB
Volume
9
Category
Article
ISSN
0043-7719

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โœฆ Synopsis

A simple automated analytical method for separation and quantitative determination of sugars from acidic and enzymatic hydrolysates of wood polysaccharides via borate complex ion exchange chromatography is described. Samples are analysed without any interferring manipulation like derivatization, deionization or deproteinization, and even sulphuric acid hydrolysates can be directly applied to the column. As little as 0.5 ~g of an individual sugar can be quantitatively measured. Examples of the separation of common monomeric sugars and mixtures of mono-and disaccharides and uronic acids are given.

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โœ Susumu Honda; Masaye Takahashi; Kazuaki Kakehi; Shigetake Ganno ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 637 KB

Biochemically important monosaccharides were rapidly separated by automated chromatography of their borate complexes on columns packed with newly developed anion-exchange resins, and sensitively detected by fluorimetric postlabeling with 2-cyanoacetamide. Optimization studies for stepwise, gradient,