✦ LIBER ✦

Rapid, automated analysis of monosaccharides by high-performance anion-exchange chromatography of borate complexes with fluorimetric detection using 2-cyanoacetamide

✍ Scribed by Susumu Honda; Masaye Takahashi; Kazuaki Kakehi; Shigetake Ganno

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 637 KB
Volume: 113
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90055-5

No coin nor oath required. For personal study only.

✦ Synopsis

Biochemically important monosaccharides were rapidly separated by automated chromatography of their borate complexes on columns packed with newly developed anion-exchange resins, and sensitively detected by fluorimetric postlabeling with 2-cyanoacetamide. Optimization studies for stepwise, gradient, and single-buffer elutions are described, and the reproducibility of the determination of such monosaccharides is discussed. A few examples of the application of this rapid, sensitive automated analysis are also presented.

📜 SIMILAR VOLUMES

Automated analysis of hexosamines by hig

Automated analysis of hexosamines by high-performance liquid chromatography with photometric and fluorimetric postcolumn labeling using 2-cyanoacetamide

✍ Susumu Honda; Tadao Konishi; Shigeo Suzuki; Masaye Takahashi; Kazuaki Kakehi; Sh 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 441 KB

Automated analysis of uronic acids by hi

Automated analysis of uronic acids by high-performance liquid chromatography with photometric and fluorimetric postcolumn labeling using 2-cyanoacetamide

✍ Susumu Honda; Shigeo Suzuki; Masaye Takahashi; Kazuaki Kakehi; Shigetake Ganno 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 522 KB

A convenient method for the rapid and sensitive automated analysis of uronic acids, based on high-performance anion-exchange chromatography on a Hitachi 2633 column and photometric as well as fluorimetric postcolumn labeling with 2-cyanoacetamide, has been developed. This method allows the simultane