𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Automated precolumn derivatization of hydrolysate amino acids using AminoTag: Automix derivatization and extraction

✍ Scribed by Terry L. Sheehan; Anton G. Mayer; Robert L. Cunico; Elisabeth Korte

Book ID
112328429
Publisher
Springer
Year
1987
Tongue
English
Weight
148 KB
Volume
327
Category
Article
ISSN
1618-2650

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Automated precolumn derivatization syste
Automated precolumn derivatization system for analyzing physiological amino acids by liquid chromatography/mass spectrometry
✍ Kazutaka Shimbo; Shintaro Kubo; Yushi Harada; Takashi Oonuki; Takefumi Yokokura; πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 435 KB

## Abstract An automated method for high‐throughput amino acid analysis, using precolumn derivatization high‐performance liquid chromatography/electrospray mass spectrometry (HPLC/ESI‐MS), was developed and evaluated. The precolumn derivatization step was performed in the reaction port of a home‐bu

Determination of amino sugars and amino
Determination of amino sugars and amino acids in glycoconjugates using precolumn derivatization with o-phthalaldehyde
✍ Friedrich Altmann πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 529 KB

This report examines the RP-HPLC separation of o-phthalaldehyde derivatives of amino acids, amino sugars, and amino sugar alcohols using either 2-mercaptoethanol or 3-mercaptopropionic acid. A method with pmol sensitivity for the analysis of N-acetylamino sugars of glycoconjugates was elaborated. Up

Fully automated amino acid analysis for
Fully automated amino acid analysis for protein and peptide hydrolysates by precolumn derivatization with 9-fluorenyl methylchloroformate and 1-aminoadamantane
✍ Barbro Gustavsson; Ingvar BetnΓ©r πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 551 KB

A fully automated precolumn derivatization method for the determination of primary and secondary amino acids using reversed-phase chromatography is described. The derivatization reagent 94uorenyl methylchloroformate is used, together with 1-aminoadamantane for reaction with excess of reagent. The de

Evaluation of conditions for the HPLC de
Evaluation of conditions for the HPLC determination of plasma amino acids using precolumn derivatization
✍ Halfpenny, A. P. ;Brown, Ph. R. πŸ“‚ Article πŸ“… 1985 πŸ› John Wiley and Sons 🌐 English βš– 440 KB πŸ‘ 1 views

## Abstract For the determination of free amino acids in plasma, the conditions for precolumn derivatization of the amino acids and the chromatographic separation were examined. The isoindole products, formed by the reaction of the primary amino acids with orthophthalaldehyde (OPA), were readily se