Fully automated amino acid analysis for protein and peptide hydrolysates by precolumn derivatization with 9-fluorenyl methylchloroformate and 1-aminoadamantane
✍ Scribed by Barbro Gustavsson; Ingvar Betnér
- Book ID
- 104145604
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 551 KB
- Volume
- 507
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A fully automated precolumn derivatization method for the determination of primary and secondary amino acids using reversed-phase chromatography is described. The derivatization reagent 94uorenyl methylchloroformate is used, together with 1-aminoadamantane for reaction with excess of reagent. The derivatization procedure is described in detail. Reproducibility data are presented and compared between the manual and the fully automated methods. Optimization strategies for separation, and differences in detection between fluorescence and UV methods are discussed. Analytical data for protein and peptide hydrolysates are compared with their sequenced data.