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Automated image processing of the colon crypt epithelium

โœ Scribed by G. Zajicek; H.P. Meinzer; D. Komitowski

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
674 KB
Volume
15
Category
Article
ISSN
0010-4809

No coin nor oath required. For personal study only.

โœฆ Synopsis

Colons of young adult Sprague-Dawley rats are embedded in paraffin, cut, and stained with Feulgen. The sections are scanned with a Quantimet-system 23 image analyzer under PDP-1 l/34 computer control. Each crypt is digitized into 192 x 700 g-bits pixels. The images are stored on magnetic tapes and processed on a PDP-15/76 computer providing the necessary features for interactive image analysis. The epithelium of each crypt image is separated, and the cells are ordered and numbered according to their position in relation to crypt origin. The location of mitotic figures is also recorded. The progenitor region outer boundary is estimated with the aid of life table analysis. The program also computes the average crypt length and the progenitor/functional ratio, and estimates the epithelial cell production rate.

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