✦ LIBER ✦

Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy

✍ Scribed by Michal Kozubek; Petr Matula; Pavel Matula; Stanislav Kozubek

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 412 KB
Volume: 64
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.20068

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The successful development of visualization techniques for live cell imaging leads to the development of suitable software for the acquisition and processing of multidimensional image data. This report compares several possible approaches to image acquisition and processing in confocal in vivo microscopy and suggests new alternatives to the published methods. Special attention is paid to spinning disk systems based either on a classical Nipkow disk or on the microlens principle. This study shows how to optimize image acquisition process in live cell studies using camera binning feature and how to perform object tracking using a new fast image registration method based on the graph theory. Microsc. Res. Tech. 64:164–175, 2004. © 2004 Wiley‐Liss, Inc.

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