Acquisition of high-resolution digital images in video microscopy: Automated image mosaicking on a desktop microcomputer
✍ Scribed by Ott, Swidbert R.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 335 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1059-910X
No coin nor oath required. For personal study only.
✦ Synopsis
For the digital processing of microscopical images, mosaicking is a prerequisite if the specimen is larger than the camera field at the necessary magnification. This study investigates the possibilities and limitations of fully automated mosaicking on a desktop computer. Cross-correlation-based frame registration was performed with high reliability if the video frames were edge-enhanced before matched filtering, and also in a reasonable time since the search for matches was restricted to pairs of consecutive frames. An environment for routine mosaicking was developed and implemented in a widely used desktop image-processing program. The software developed during this study has been released to the public domain.