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Automated, high-throughput platform for protein solubility screening using a split-GFP system

✍ Scribed by Pawel Listwan; Thomas C. Terwilliger; Geoffrey S. Waldo


Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
406 KB
Volume
10
Category
Article
ISSN
1345-711X

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The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the luciferase gene, in vitro translation of M-hirudin is highly robust against changes in concentrations of K Ψ‰ (and Rb Ψ‰ ). In addition, M-hirud