Automated direct determination of chromium in blood and urine by electrothermal atomic absorption spectrometry

✍ Scribed by J.J. McAughey; N.J. Smith

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 707 KB
• Volume: 193
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86146-2

✦ Synopsis

A simple direct procedure for the determination of chromium in whole blood and urine by graphite-furnace atomic absorption spectrometry is described. Whole blood samples are diluted with 0.1% Triton-X solution before injection, whereas urine samples are injected directly. Calibration is done by direct comparison against matrix-matched standards. Between-run precision is 6.4% at 154 nmol 1-l for urine and 3.6% at 142 nmol 1-l for blood. The detection limits are 3.8 nmol 1-l for urine and 11.5 nmol 1-l for blood, each for a 20+1 sample. The calibration range extends up to 770 nmol 1-l for both blood and urine. This allows the determination of chromium in both occupationally exposed and unexposed groups. The graphite-furnace conditions for each matrix are similar. Elimination of sample pretreatment minimizes the risk of contamination and allows a rapid sample throughput of 50-60 samples per day. The methods described are particularly suited for the screening and surveying of populations occupationally exposed to chromium.