Ninhydrin-ferric reagent reacts highly and specifically with lysine at a pH value of 1.0. Ferric ion inhibits the reaction of ninhydrin with proline, ornithine, glycine, arginine, and histidine. This method can be used to determine a lysine sample of high concentration without dilution. It has a lin
Automated determination of lysine by colorimetric method with ninhydrin
โ Scribed by Albert Bordons
- Publisher
- Springer Netherlands
- Year
- 1986
- Tongue
- English
- Weight
- 236 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0141-5492
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Attempts have been made to develop a simpler and more specific method for determining free a-amino acids in chemical and biological systems. The results obtained are presented in this paper. METHODS ## AND RESULTS Reagents: 0.5 M citrate (Na+) buffer was prepared from analyticalgrade citric aci