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Automated assay for δ-aminolevulinic acid dehydratase

✍ Scribed by M.G. Gore; P.M. Jordan; A.G. Chaudhry

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
375 KB
Volume
87
Category
Article
ISSN
0003-2697

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✦ Synopsis

An automated procedure for the assay of semipurified and purified 8-aminolevulinic acid dehydratase (EC 4.2.1.24) has been developed based on the manual spectrophotometric method of Mauzerall and Granick (1). This method has been used to determine (a) the enzyme activity in various samples during enzyme-purification procedures, (b) the dependence of initial rates of catalysis for a fixed enzyme concentration on the substrate concentration, and(c) the variation in the level of enzyme activity during and after chemical modification.

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