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Automated analysis of polyethylene glycol-induced inhibition of P-glycoprotein activity in vitro

โœ Scribed by Erin D. Hugger; Clayton J. Cole; Thomas J. Raub; Philip S. Burton; Ronald T. Borchardt

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
89 KB
Volume
92
Category
Article
ISSN
0022-3549

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โœฆ Synopsis

Previous studies in our laboratories have shown that commonly used polyethoxylated pharmaceutical excipients inhibit P-glycoprotein activity in cell culture models of the intestinal mucosa. The results presented in this technical note show that the TECAN Genesis robotic workstation can be utilized to automate cellular transport studies for evaluating excipient effects on P-glycoprotein activity in vitro and for estimating the permeation of drug-like molecules across cell monolayers.

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