Autocrine control of human meningioma proliferation: Secretion of platelet-derived growth-factor-like molecules

✍ Scribed by Eric F. Adams; T. Todo; U. M. H. Schrell; P. Thierauf; M. C. White; R. Fahlbusch

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 505 KB
• Volume: 49
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910490315

✦ Synopsis

We have used cell-culture techniques to investigate growthfactor production by human meningioma cells. Meningioma tissue was dispersed with collagenase and the cells grown to high density in tissue-culture flasks. The cultures were used to generate conditioned medium (MEN-CM), which was used to cultivate IMR32 cells (a human neuroblastoma line) and freshly dispersed primary meningioma cells. MEN-CM profoundly stimulated the in vitro growth of both IMR32 and meningioma cells. In addition, H'-thymidine uptake by cultured meningioma cells was increased in a dose-dependent manner by varying concentrations of MEN-CM. A neutralizing anti-body against plateletderived growth factor (PDGF) completely abolished the stimulatory effects of MEN-CM, whereas an antibody against TGF-cy was without effect. The mitogenic activity of MEN-CM, as assayed by promotion of H'-thymidine uptake by cultured meningioma cells, eluted from a Sephadex G-I00 column in 3 peaks corresponding to molecular weights of 2 150, 56 and 28 kDa. Our results show that proliferation of human meningiomas may be under autocrine control via secretion of PDGF-like molecules.