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Asymmetric distribution of Concanavalin A binding sites on yeast plasmalemma and vacuolar membrane

✍ Scribed by Thomas Boller; Mathias Dürr; Andres Wiemken


Book ID
104770030
Publisher
Springer
Year
1976
Tongue
English
Weight
554 KB
Volume
109
Category
Article
ISSN
0302-8933

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✦ Synopsis


Isolated vacuoles of Saccharomyces cerevisiae did not bind Concanavalin A (labelled with tritium or with a fluorescent dye) unless the vacuoles were rendered permeable and their inner membrane surface made accessible. Yeast protoplasts, on the other hand, bound large amounts of Concanavalin A on their surface, and the number of binding sites was not increased after a gentle lysis expected to expose also the inner surface of the plasmalemma. It is concluded that both the plasmalemma and the vacuolar membrane carry Concanavalin A binding sites exclusively on the surface opposite to the cytoplasmic matrix.


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