Asymmetric distribution of Concanavalin A binding sites on yeast plasmalemma and vacuolar membrane
✍ Scribed by Thomas Boller; Mathias Dürr; Andres Wiemken
- Book ID
- 104770030
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 554 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
Isolated vacuoles of Saccharomyces cerevisiae did not bind Concanavalin A (labelled with tritium or with a fluorescent dye) unless the vacuoles were rendered permeable and their inner membrane surface made accessible. Yeast protoplasts, on the other hand, bound large amounts of Concanavalin A on their surface, and the number of binding sites was not increased after a gentle lysis expected to expose also the inner surface of the plasmalemma. It is concluded that both the plasmalemma and the vacuolar membrane carry Concanavalin A binding sites exclusively on the surface opposite to the cytoplasmic matrix.
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