✦ LIBER ✦

Association of tyrosine protein kinase activity with mitochondria in human fibroblasts

✍ Scribed by Giuseppe Piedimonte; Lucia Silvotti; Angelo F. Borghetti; Solange Chamaret; Luc Montagnier

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 648 KB
Volume: 32
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240320204

No coin nor oath required. For personal study only.

✦ Synopsis

A tyrosine protein kinase activity has been detected in the mitochondrial fraction purified from human fibroblasts. By enzymatic and sedimentation analysis this activity appeared to be localized in the mitochondrial outer membrane. Mitochondrial tyrosine phosphorylation was strictly dependent on the presence of Mn2+ ions. An inverse relationship between cell proliferation and mitochondrial protein phosphorylation on tyrosine residues has been found: a marked increase in the mitochondrial tyrosine kinase activity occurred when a significant reduction in the growth rate followed serum step-down. In mitochondria purified from resting cells, a protein band with apparent molecular weight of 50 kd appeared to be phosphorylated on tyrosine.

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