β Scribed by Hug, H. ;Fiechter, A.
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Analytical problems in microbial experiments using hydrocarbons as the carbon source are notorious. Several procedures for assays of biomass and substrate have been evaluated. Reliable results for dry matter determination were obtained by centrifugation of the samples in presence of solvents.
Extraction of hydrocarbons was effected by strong mixing of the emulsion with the solvent. The extract is then submitted to gas liquid chromatography (GLC).
The procedures for hydrocarbon determination were worked out with model suspensions and evaluated in actual cultivations. Typical batch data from Candida tropicalis experiments showed only slight deviations from a 100~ carbon balance.
Yields (g biomass per g substrate) were about 112~ . 4. Loss of carbon atoms in the form of CO~ was 10--20~ lower than reported in literature.
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In cell free extracts prepared from protoplasts of n-tetradecane grown cells of Candida tropicalis we have found an ATP and NAD+ dependent alkane-dehydrogenase, an alcohol-dehydrogenase, an aldehyde-dehydrogenase and acyl-CoA synthetases. The study of these enzymes and their regulation allows us to
## Abstract The utilization of __n__βhexadecane by __Candida lipolytica__ (strain 10) was studied with respect to the lipid content, phospholipid and fatty acid profiles resulting at various growth times. Thin layer chromatography of the lipid extracts showed quantitative changes in the different l