Assignment of the gene for dipeptidase 2 toMus musculuschromosome 18 by somatic cell hybridization
β Scribed by Leslie A. Leinwand; Frank H. Ruddle
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 312 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2928
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β¦ Synopsis
Evidence is presented for the assignment of the gene for dipeptidase 2 to Mus musculus chromosome 18 by synteny testing and karyotypic analysis of Chinese hamster x mouse somatic cell hybrid clones. DIP-2 and chromosome 18 were expressed concordantly in 24/24 clones examined (ten primary clones and 14 secondary clones). Synteny testing indicated that DIP-2 was not expressed concordantly with the expression of any marker enzymes.
π SIMILAR VOLUMES
A specific enzyme immunoassay of uroporphyrinogen decarboxylase was developed and applied to the detection of the human enzyme in man-rodent somatic cell hybrids. This method allowed to assign the gene for uroporphyrinogen decarboxylase to human chromosome 1.
Human/hamster hybrid cell cultures were examined for the presence of ARSA and other marker enzymes. Many of these hybrids were also analyzed for human chromosomes. Our results confirm the assignment of ARSA to chromosome 22.
Evidence is presented for the assignment of the gene for glyoxylase I to mouse chromosome 17 using mouse x Chinese hamster somatic cell hybrids. GLO I was not expressed concordantly with any known marker enzymes which represented 11 linkage groups. The presence of chromosome 17 and expression of GLO