Assignment of the 1H-NMR spectrum of a lac repressor headpiece-operator complex in H2O and identification of NOEs : Consequences for protein-DNA interaction

DNA–protein interaction in solution: NMR

DNA–protein interaction in solution: NMR studies of the 13C-site-specifically labelled Lac operator–Lac repressor complex

✍ Gérard Lancelot; Jean-Claude Maurizot; Marcel Chassignol; Nguyeng T. Thuong; Luc 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB

## Abstract Carbon‐13 and proton resonance assignments for a DNA fragment complexed with its regulatory protein were obtain with the use of a selectively ^13^C‐labelled oligonucleotide. Data obtained on the Lac operator–Lac repressor complex from the HMQC–NOESY maps of the free and complexed Lac op