Assignment of all 31P resonances of a 16 base-pair DNA duplex, Sd(CTGCTCACTTTCCAGG)Y Sd(CCTGGAAAGTGAGCAG)Y, related to the DNA KB site of the HIV-1 LTR, was determined by 2D heteronuclear inverse NMR spectroscopy (HSQC-TOCSY and heteronuclear COSY). 'J(H3'-P) coupling constants for most of the oligo
Assignment of phosphate groups and determination of P,H coupling constants in the DNA fragment d(CGTACG) by 31P 2D NMR experiments
✍ Scribed by Enzio Ragg; Rosanna Mondell; Anna Garbesi; Francesco P. Colonna; Carlo Battistini; Sergio Vioglio
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 488 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0749-1581
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## Abstract The indirect determination of PP coupling constants and their relative signs in some symmetrical diphosphonates by ^31^P^13^C 2D NMR is introduced. Advantages and disadvantages of this method and others for the determination of the coupling constants of chemically equivalent nuclei ar
A two-dimensional { 31 P} spin-echo-difference constant-time [ 13 C, 1 H]-HMQC experiment (2D { 31 P}-sedct-[ 13 C, 1 H]-HMQC) is introduced for measurements of 3 J C4P and 3 J H3P scalar couplings in large 13 C-labeled nucleic acids and in DNA-protein complexes. This experiment makes use of the fac