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Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

✍ Scribed by Bernard Quéméner; Juan Carlos Cabrera Pino; Marie-Christine Ralet; Estelle Bonnin; Jean-François Thibault

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
163 KB
Volume
38
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI‐ITMS). The ^18^O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross‐ring cleavages. The collisional‐induced dissociation of the C~i~ and Z~j~ fragment ions through sequential MS^n^ experiments always displayed ^0, 2^A‐type cross‐ring cleavage ions which were related to C~2~H~4~O~2~ losses. These ^0, 2^A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O‐2 and/or O‐3 of the acetylated galacturonic acid residues. Copyright © 2003 John Wiley & Sons, Ltd.

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