A putative Ets site with a core of GGAA located at nt -88 to -85 of the rat ornithine decarboxylase (ODC) gene was characterized by site-directed mutagenesis and transient expression assays. Mutation of this site, when in pODClux2m, which contains a cluster of four Sp1-binding sites, resulted in a 2
Assignment of a gene encoding ornithine decarboxylase to the proximal region of chromosome 12 in the mouse
β Scribed by Franklin G. Berger
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 404 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Brief Data Reports been mapped to Chr 3 by human-hamster somatic cell hybrid analysis. We localized the mouse Trh gene on Chr 6 in a region with homology to the human Chr 3 [6]. There are no known mouse mutants in this region that exhibit hypothroidism or growth insufficiency.
## Method of mapping: 113 animals from the backcross (DP-BB β’ DR-BB)F 1 β’ DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC
The human 8.5 H probe was isolated from a human cerebellum cDNA library with a probe corresponding to the coding region of the murine 8.5 M cDNA. This cDNA isolated from a routine cDNA library constructed from newborn cerebral hemispheres was selected because of its strong expression in embryonic ne