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Assessment of small polymorphisms in defined human collagen gene segments

✍ Scribed by Maria Sippola-Thiele; G. C. Tromp; D. J. Prockop; F. Ramirez

Publisher: Springer
Year: 1986
Tongue: English
Weight: 455 KB
Volume: 72
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00291887

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✦ Synopsis

Restriction fragment length polymorphisms (RFLPs) greater than 1.5 kb in size have been identified in all but one of the human fibrillar collagen genes. However, the number of informative RFLPs for these genes is still limited. Here we present the conjunct use of two techniques for the assessment of small length variations within defined segments of the genome. This strategy has the potential to be used for the allelic exclusion of cloned genomic fragments prior to sequencing. Moreover it can be a useful and sensitive tool to determine if genetic linkage exists between abnormal phenotypes and loci where conventional Southern blotting analysis has failed to detect informative RFLPs.

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