Antiserum against purified rat liver microaomal epoxide hydrolase was produced in the rabbit. We developed an enzyme-linked immunoaorbent assay which is reliable with regard to its analytical criteria. The concentration of epoxide hydrolase was measured in liver microaomes of control rats and animal
Assessment of rat liver microsomal epoxide hydrolase as a marker of hepatocarcinogenesis
β Scribed by Donald E. Kizer; Joseph A. Clouse; David P. Ringer; Olivia Hanson-Painton; Alfin D. Vaz; Ram B. Palakodety; Martin J. Griffin
- Book ID
- 115778535
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 527 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-2952
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Pur i f ied epox ide hydrolase was immobilized by covalent binding to Sephadex G-150 activated with 1,l' carbonyl diimidazole under mild conditions Kmapp values of free and immobilized epoxide hydrolase were 0.5~ M and 2~ M respectively towards benzo(a)p rene-4,5-oxide,-YherEys Vmaxapp was decreased
Microsomal epoxide hydrolase (mEH) is inducible by a number of xenobiotics. Induction of mEH by certain chemopreventive agents may implicate the protective effect. In contrast, many of carcinogenic agents also induce the enzyme. The hepatotoxicity and mEH expression by methylthiazoles, which are inc