Hereditary non-polyposis colorectal cancer (HNPCC) is a dominantly inherited syndrome linked to DNA-mismatch-repair (MMR) gene defects, which also account for microsatellite instability (MSI) in tumour tissues. Diagnosis is based mainly on family history, according to widely accepted criteria (Amste
Assessment of microsatellite instability in colorectal cancer patients from Brazil
β Scribed by Sinara M. O. Leite; Karina B. Gomes; Victor C. Pardini; Alessandro C. S. Ferreira; Vanessa C. Oliveira; Geraldo M. G. Cruz
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 189 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0301-4851
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## BACKGROUND. Microsatellite instability (MI) has been reported in some sporadic colon tumors and in cases of hereditary nonpolyposis colorectal cancer (HNPCC). The criteria for HNPCC have not been fully defined, and clinical criteria are used to identify as many HNPCC patients as possible. To cl
The reproducibility of microsatellite instability from different regions of the same sporadic colon cancer has not been addressed. We therefore microdissected and extracted DNA from three to nine separate regions of 13 highly unstable sporadic colon cancers. Each region was then evaluated by polymer