Assessment of methanolysis for the determination of sugars in pectins

✍ Scribed by Bernard Quemener; Jean-François Thibault

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 700 KB
• Volume: 206
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(90)80067-d

✦ Synopsis

A procedure for the determination of galacturonic acid and the main neutral sugars in pectins involves enzymic hydrolysis followed by methanolysis and h.p.1.c. The usefulness of this method was demonstrated by comparison of the results obtained by (I) methanolysis in methanolic M HCI without enzymic prehydrolysis, (2) methanolysis in methanolic 72% H,SO, with pretreatment for 3 h with aqueous 72% H2S04, (3) calorimetric determination of galacturonic acid, and ( 4) g.1.c. of the alditol acetates of the neutral sugars released by acid hydrolysis under various conditions. EXPERIMENTAL General. -The h.p.1.c. system involved a Waters 590 programmable pump, an Erma ERG3310 degasser, a Touzart/Matignon pulsation dumper, a Valco C 6 W