Assembly and Mixing of Two-Stage Aqueous Two-Phase Protein Extractions
β Scribed by Jonathan E. Bleier; Esther H.-J. Kim; Xiao Dong Chen
- Book ID
- 109386267
- Publisher
- American Institute of Chemical Engineers
- Year
- 2001
- Tongue
- English
- Weight
- 209 KB
- Volume
- 17
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
An iminodiacetic acid derivative of poly(ethylene glycol) (PEG-IDA) that chelates metal cations has been synthesized and used to extract proteins in metal affinity aqueous two-phase PEG/ dextran systems. With less than 1% of the PEG substituted with chelated copper, partition coefficients are shown
## Abstract **BACKGROUND:** Aqueous twoβphase extraction (ATPE) has many advantages as an efficient, inexpensive largeβscale liquidβliquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to t