Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions

✍ Scribed by Wayne F. Beyer Jr.; Irwin Fridovich

• Book ID: 102988661
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 610 KB
• Volume: 161
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90489-1

✦ Synopsis

Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.