## Abstract A holder for a 12 cm long capillary was designed for scanning LIF detection of CIEF. The polyimide coat of a fusedβsilica capillary has been removed, and 1.5 mm diameter flanges have been attached near both ends. The holder is fixed on the stage of a fluorescence microscope __via__ a tr
β¦ LIBER β¦
Assay of trypsin activity by capillary isoelectric focusing with laser-induced fluorescence detection
β Scribed by Professor Kiyohito Shimura; Ken-ichi Kasai; Hiroyuki Matsumoto
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 561 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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## Abstract CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2βD separation method for proteins. In this method, two capillaries are joined through a bufferβfilled interface. Separate power supplies control the potential at the injection end of the first capillary and at the
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