A method has been developed for the simultaneous and rapid analysis of the 3-hydroxypyridinium crosslinks of mature collagen, pyridinoline and deoxypyridinoline, in samples of urine and tissue. After hydrolysis in 6 M HCl, samples were prefractionated by partition chromatography using columns packed
Assay of pyridinium crosslinks in serum using narrow-bore ion-paired reversed-phase high-performance liquid chromatography
โ Scribed by Ian James; Claire Crowley; David Perrett
- Publisher
- Elsevier Science
- Year
- 1993
- Weight
- 679 KB
- Volume
- 612
- Category
- Article
- ISSN
- 0378-4347
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A fully automated method for assaying the collagen crosslinking amino acids, pyridinoline and deoxypyridinoline, in human urine samples or tissue hydrolysates is described. Samples were processed using a Gilson ASPEC system with solid-phase extraction of the crosslinks on columns containing 100 mg o
An ion-pair reversed-phase high-performance liquid chromatographic method is presented for the determination of adriamycin and adriamycinol concentrations in rat serum and tissues. The scheme for developing the chromatographic conditions is discussed. A good separation of adriamycinol and adriamycin