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Assay of nucleoside-diphosphate kinase activity by the coupled nucleotidyltransferase

✍ Scribed by Norimitsu L. Sato

Book ID
102629361
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
150 KB
Volume
166
Category
Article
ISSN
0003-2697

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✦ Synopsis

poly(A) polymerase, tRNA nucleotidyltrans-2. Berg, P.. and Joclih. W. K. (1953) &'arr,w IL~wtlonj ferase, etc. Although it was possible to detect 172,100X. the NDP-kinase activity of the crude cyto-3. Reddy. G. P. U., and Pardce. A. B. ( 1980) Proc,. plasmic fraction by the present assay h:atl. .kad. Si. L'S.1 77. 33 I?.

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A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for te