✦ LIBER ✦
Assay of Nucleoside Diphosphate Kinase in Microtiter Plates Using a Peroxidase-Coupled Method
✍ Scribed by I. Lascu; K. Leblay; M.L. Lacombe; E. Presecan; M. Veron
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 220 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes. 1993 Academic Press, Inc.