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Assay of hypoglycin in biological samples

✍ Scribed by Eccleston A. Kean

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
327 KB
Volume
90
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and rapid calorimetric method, applicable to extracts of biological samples, is reported for measuring the toxic amino acid L-a-amino-fi-methylenecyclopropylpropionic acid (hypoglycin). By fluorimetry, as little as 10 nmol (0.14 pg) can be assayed. The amino acid is made to yield formaldehyde by reaction with permanganate under defined conditions, and from the aldehyde, 35 diacetyl-4-dihydrolutidine is produced and measured.

EXPERIMENTAL PROCEDURE Samples analysed. Standards prepared from chromatographically pure hypoglycin (7) dissolved in 0.01 N HCl were used to monitor the pro-

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