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Assay of cysteine conjugate β-lyase activity with S-(2-benzothiazolyl)cysteine as the substrate

✍ Scribed by David R. Dohn; M.W. Anders


Book ID
102984670
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
663 KB
Volume
120
Category
Article
ISSN
0003-2697

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✍ Gerard J. Stijntjes; Johan M. te Koppele; Nico P.E. Vermeulen 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 936 KB

An HPLC-fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate beta-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid,