High-performance liquid chromatography-f
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Gerard J. Stijntjes; Johan M. te Koppele; Nico P.E. Vermeulen
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Article
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1992
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Elsevier Science
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English
⚖ 936 KB
An HPLC-fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate beta-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid,