Assay in situ of yeast enzymes involved in protohaem synthesis
β Scribed by Volland, Christiane; Labbe-Bois, Rosine; Labbe, Pierre
- Book ID
- 122774885
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 380 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0300-9084
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A very simple method that allows for the rapid in situ assay of enzyme activity in yeast is described. Single colonies are collected on sticks (or glass micropipets), or multiple colonies are collected on sandpaper, and crushed onto nitrocellulose filters. The tilters in turn are stained for the enz
The yeast, Kluyveromyces fragilis was permeabilized to a number of low-molecular-weight substrates using digitonin. The activities of intracellular yeast enzymes, viz., alcohol dehydrogenase (ADH), beta-galactosidase, glucose-6-phosphate dehydrogenase, aspartase, and hexokinase were found to be much