Artifact formation during transmethylation of lipid peroxides

✍ Scribed by Franz Ulberth; Wolfgang Kamptner

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 313 KB
• Volume: 203
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(92)90039-a

✦ Synopsis

By-product formation during base-catalyzed transesterification of lipid peroxides was observed in model experiments. A partially oxidized linoleic acid methyl ester as well as purified hydroxylinoleates served as test compounds. By-products formed during a simulated transmethylation of purified hydroxylinoleates were separated from the main components by means of thin-layer chromatography. The loss attributable to these by-products amounted to 10%. Oxygenated fatty acid derivatives were completely destroyed by acid-catalyzed transmethylation. Catalytic hydrogenation prior to base-catalyzed transmethylation proved to be a simple means to minimize side-product formation. By using this technique the yield of hydroxystearates from a partially autoxidized linoleic acid methyl ester preparation was improved significantly.