✦ LIBER ✦

Artefactual effects of oxygen on cell culture models of cellular senescence and stem cell biology

✍ Scribed by Olivier Toussaint; Geoffroy Weemaels; Florence Debacq-Chainiaux; Karin Scharffetter-Kochanek; Meinhard Wlaschek

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 109 KB
Volume: 226
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.22416

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In life sciences, modelling of the in vivo conditions using in vitro models is an important tool to generate knowledge. Although aerobic organisms including mammals depend on accurate oxygen tension, mimicking physiological conditions in cell culture experiments is not very common. Due to the need for simple technical and experimental design, the requirement for simulating the in vivo oxygen tension parameters has been neglected over long time. Fortunately, due to increasing knowledge in recent years the attention has shifted towards this scientific demand. In this short review, we summarize data substantiating the necessity to adequately mimic physiological oxygen tension using cell culture models in life science research. J. Cell. Physiol. 226: 315–321, 2011. © 2010 Wiley‐Liss, Inc.

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