𝔖 Bobbio Scriptorium
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Aromatic amino acid biosynthesis inAlcaligenes eutrophus H 16

✍ Scribed by C. G. Friedrich; Bärbel Friedrich; H. G. Schlegel

Book ID
104777903
Publisher
Springer
Year
1976
Tongue
English
Weight
717 KB
Volume
107
Category
Article
ISSN
0302-8933

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✦ Synopsis

Properties and regulation of anthranilate synthase from Alcaligenes eutrophus H 16 were investigated. Anthranilate synthase was partially purified from crude extracts by affinity chromatography on tryptophan-substituted Sepharose, and was used for kinetic measurements. During the purification procedure the enzyme was stabilized by 50 mM L-glutamine or during chromatography on DEAE- cellulose and Sephadex G-200 with 30% glucerol, respectively.

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✍ Mi Kyoung Kim; In Young Lee; Young Hoon Park 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 308 KB

Additon of pyruvate or leucine was found to be efficient for increasing the intracellular ratios of NADH/NAD and NADPH/NADP while reducing the coenzyme A concentration during the cultivation of Alcaligenes eutrophus. Poly-/3 -hydroxybutyrate (PHB) accumulation was enhanced more than 2-fold since met