✦ LIBER ✦
Metabolites and amino acids affecting cellular cofactor concentrations and Poly-β-hydroxybutyrate biosynthesis inAlcaligenes eutrophus
✍ Scribed by Mi Kyoung Kim; In Young Lee; Young Hoon Park
- Book ID
- 104638487
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 308 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
✦ Synopsis
Additon of pyruvate or leucine was found to be efficient for increasing the intracellular ratios of NADH/NAD and NADPH/NADP while reducing the coenzyme A concentration during the cultivation of Alcaligenes eutrophus. Poly-/3 -hydroxybutyrate (PHB) accumulation was enhanced more than 2-fold since metabolic flux of acetyl-CoA into PHB synthetic pathway could be facilitated by the changes of the cofactor concentrations.