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Approaching the physiological functions of penicillin-bindingproteins in Escherichia coli

✍ Scribed by Kevin D Young


Book ID
117456541
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
32 KB
Volume
83
Category
Article
ISSN
0300-9084

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation