Approaches to DNA Mutagenesis: An Overview
β Scribed by Michael Mingfu Ling; Brian H. Robinson
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 296 KB
- Volume
- 254
- Category
- Article
- ISSN
- 0003-2697
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DNA repair systems act to maintain genome integrity in the face of replication errors, environmental insults, and the cumulative effects of age. More than 70 human genes directly involved in the five major pathways of DNA repair have been described, including chromosomal location and cDNA sequence.
## Abstract Alanine scanning mutagenesis of proteinβprotein interfacial residues can be applied to a wide variety of protein complexes to understand the structural and energetic characteristics of the hotβspots. Binding free energies have been estimated with reasonable accuracy with empirical metho