Approach to improve compound recovery in a high-throughput Caco-2 permeability assay supported by liquid chromatography–tandem mass spectrometry

Validation of higher-throughput high-per

Validation of higher-throughput high-performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry assays to conduct cytochrome P450s CYP2D6 and CYP3A4 enzyme inhibition studies in human liver microsomes

✍ Inhou Chu; Leonard Favreau; Tony Soares; Chin-chung Lin; Amin A. Nomeir 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB 👁 2 views

In the early stage of drug discovery, thousands of new chemical entities (NCEs) may be screened before a single drug candidate can be identified for development. In order to accelerate the drug discovery process, we have developed higher-throughput enzyme assays to evaluate the inhibition of cytochr