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Applications of heteroduplex analysis for mutation detection in disease genes

✍ Scribed by Damjan Glavac; Michael Dean

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 691 KB
Volume: 6
Category: Article
ISSN: 1059-7794
DOI: 10.1002/humu.1380060402

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✦ Synopsis

Communicated by R. G. H. Cotton Double-stranded heteroduplex molecules that form between a mutant and wild-type D N A strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the H A method have been developed that increase the sensitivity of detection of single-base pair alterations. *O 1995 Witey-Liss, Inc.

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