Applications of circular dichroism (CD) for structural analysis of proteins: qualification of near- and far-UV CD for protein higher order structural analysis
โ Scribed by Cynthia H. Li; Xichdao Nguyen; Linda Narhi; Letha Chemmalil; Edward Towers; Salman Muzammil; John Gabrielson; Yijia Jiang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 553 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0022-3549
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Inverse circular dichroism (CD) spectra are presented for each of the five major secondary structures of proteins: alpha-helix, antiparallel and parallel beta-sheet, beta-turn, and other (random) structures. The fraction of the each secondary structure in a protein is predicted by forming the dot pr
The CD spectra for 10 proteins with known secondary structure have been extended from 178 to 168 nm. Combined with the data for 6 other proteins investigated previously, this produces a basis set of 16 proteins, which can be used to analyze CD spectra for secondary structure. Extending the spectra a