Analysis of protein circular dichroism spectra for secondary structure using a simple matrix multiplication

✍ Scribed by Larry A. Compton; W.Curtis Johnson Jr.

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 960 KB
• Volume: 155
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90241-1

✦ Synopsis

Inverse circular dichroism (CD) spectra are presented for each of the five major secondary structures of proteins: alpha-helix, antiparallel and parallel beta-sheet, beta-turn, and other (random) structures. The fraction of the each secondary structure in a protein is predicted by forming the dot product of the corresponding inverse CD spectrum, expressed as a vector, with the CD spectrum of the protein digitized in the same way. We show how this method is based on the construction of the generalized inverse from the singular value decomposition of a set of CD spectra corresponding to proteins whose secondary structures are known from X-ray crystallography. These inverse spectra compute secondary structure directly from protein CD spectra without resorting to least-squares fitting and standard matrix inversion techniques. In addition, spectra corresponding to the individual secondary structures, analogous to the CD spectra of synthetic polypeptides, are generated from the five most significant CD eigenvectors.