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Applications of a post-column fluorigenic reaction in liquid chromatography for the determination of glucose and fructose in biological matrices

✍ Scribed by A. Coquet; J.-L. Veuthey; W. Haerdi; R. Degli Agosti

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
773 KB
Volume
252
Category
Article
ISSN
0003-2670

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✦ Synopsis

A post-column fluorigenic reaction with benzamidine coupled to liquid chromatographic separation was used for the determination of reducing carbohydrates in three,complex biological samples. This method allows the simultanmus determination of the different reducing sugars, which,:-epresents an improvement over batch enzymatic tests where in most instances only single monosaccharides can be determined. This selective liquid chromatographic method has a broad linear range. The method was validated by simultaneous analyses with a specific enzymatic test for glucose. Results are presented for the determination of glucose in human serum and mustard plants and glucose and fructose in white wines.

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